FIGURE 3.

CMC diminishes host acute immune response of hepatic adenovirus infection. Ad‐GFP with (GFP + CMC group) or without (GFP group) 1.3% (wt/vol, in PBS) CMC in 30 μl total volume of PBS were intrahepatically injected into C57BL/6J mice. The mice were sacrificed at 6, 24, and 72 h after injection, respectively. The retrieved liver tissues were subjected to H&E staining. Representative inflammatory cells are indicated with yellow arrows (×400) (a). The retrieved liver tissues were further subjected to IHC staining with antibodies against CD45, CD54, CD40L, CD3D, TNFα, and IL1β (b). Representative positively stained cells are indicated with red arrows (×400). Representative results are shown