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In vitro experiments to verify the anchoring functions of map‐PD‐L1. (a) Ability of map‐PD‐L1 to bind to rgEC, as analyzed by confocal laser scanning microscope. (b) Flow cytometry analysis of factors affecting map‐PD‐L1 binding to rgEC, including map‐PD‐L1 concentration, incubation time, and temperature. map‐PD‐L1, membrane‐anchored‐protein PD‐L1; rgEC, rat glomerular endothelial cell
