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. 2022 Aug 6;7(3):e10387. doi: 10.1002/btm2.10387

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© 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Comparison of manual and automated process protocols. (a) Sequence diagram of the manual and automated processes. The general protocol for the process is described with a comparison of the manual and automated processes. (b) Photographs of the manual bioreactor set up using filtered bottles, peristaltic pump and sterile welder. (c) Automated bioreactor set up within the AUTOSTEM platform, showing the bioreactors connected to the control units and a pumping station using both positive pressure and peristaltic pumps.