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. 2022 Aug 6;7(3):e10387. doi: 10.1002/btm2.10387

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© 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Cell quality assessment postexpansion in the manual and automated bioreactor systems. (a) Oil Red O staining to assess adipogenic differentiation. Scale bars, 200 μm. (b) Alizarin Red staining to assess osteogenic differentiation. Scale bars, 500 μm. (c) GAG/DNA (μg/μg) content for chondrogenic differentiation quantification. (d) Quantification of adipogenic differentiation by extraction of Oil Red O. (e) Quantification of osteogenic differentiation by calcium concentration measurement (μg/ml). (f) Cell surface marker expression assessed by flow cytometry. Data shown as mean ± SD, n = 3.