Table 7.
Effects of graded levels of dietary beta-sitosterol supplementation on the hepatic antioxidant status of broiler chickens.
| Items1 | Beta-sitosterol level (mg/kg) |
SEM2 |
P-values |
||||||
|---|---|---|---|---|---|---|---|---|---|
| 0 | 25 | 50 | 75 | 100 | ANOVA3 | Linear | Quadratic | ||
| 14 d | |||||||||
| MDA (nmol/mg protein) | 0.48 | 0.43 | 0.41 | 0.60 | 0.33 | 0.03 | 0.073 | 0.571 | 0.344 |
| GSH (mg/g protein) | 4.11 | 3.46 | 3.66 | 4.41 | 4.61 | 0.16 | 0.085 | 0.064 | 0.072 |
| SOD (U/mg protein) | 112.80 | 107.11 | 109.15 | 111.62 | 110.71 | 1.62 | 0.850 | 0.979 | 0.490 |
| GSH-Px (U/mg protein) | 41.83 | 44.91 | 35.60 | 41.58 | 45.88 | 1.56 | 0.260 | 0.659 | 0.174 |
| CAT (U/mg protein) | 4.69 | 6.73 | 5.89 | 6.04 | 6.76 | 0.26 | 0.062 | 0.048 | 0.411 |
| 21 d | |||||||||
| MDA (nmol/mg protein) | 0.57a | 0.27b | 0.61a | 0.31b | 0.24b | 0.04 | <0.001 | <0.001 | 0.349 |
| GSH (mg/g protein) | 2.92 | 2.90 | 3.03 | 2.51 | 3.24 | 0.09 | 0.126 | 0.680 | 0.244 |
| SOD (U/mg protein) | 75.02 | 87.80 | 93.45 | 82.24 | 88.50 | 2.40 | 0.136 | 0.193 | 0.127 |
| GSH-Px (U/mg protein) | 46.30 | 53.34 | 49.29 | 46.57 | 48.65 | 1.47 | 0.593 | 0.847 | 0.501 |
| CAT (U/mg protein) | 9.75 | 10.54 | 11.47 | 9.83 | 9.66 | 0.34 | 0.430 | 0.718 | 0.132 |
a-b
Means within a row with different superscripts are different at P < 0.05.
1
CAT, catalase; GSH, reduced form of glutathione; GSH-Px, Glutathione peroxidase; MDA, malondialdehyde; SOD, superoxide dismutase.
2
SEM, standard error of the mean (n = 6).
3
ANOVA, analysis of variance.