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. 2022 Sep 12;10(9):e005189. doi: 10.1136/jitc-2022-005189

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© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Composition, phenotype and genotype of CAR T cells from automated manufacturing runs. (A) Cellular composition was analyzed before and after enrichment on day 0 as well as in the final cell product using flow cytometry. (B) CD4/CD8 ratio was analyzed before and after enrichment as well as after harvest. (C) T cell immunophenotype in final cell product. T cells were stained with antibodies against CD45RO, CD62L and CD95 and frequency of naïve T cells (T_n), stem-cell memory T cells (T_scm), central memory T cells (T_cm), effector memory T cells (T_em), and CD45-RA⁺ effector memory T cells (T_emra) was assessed in the final cell product by flow cytometric analysis. (D) Vector copy numbers were analyzed from final cell products (CAR T, N=3) or mock T cells (control, N=2). (E) Gene expression profiles of cells from final cell product (N=4) were compared by Nanostring analysis to that of freshly isolated T cells (N=10). (B, C) Show mean±SD from N=3 donors.