Supplemental Figure S7.

Histologic scoring after long-term fast-food diet (FFD). A: Representative picrosirius red staining of wild-type (WT) and hepatocyte-specific Slc25a34 knockout (Slc-HKO) livers after long-term chow and FFD. B: Histologic scoring of steatosis, lobular inflammation, hepatocyte ballooning, and fibrosis. Steatosis score refers to the surface area evaluated on low- to medium-power examination. Lobular inflammation refers to the number of foci as observed at 200× magnification. Ballooning refers to ballooned hepatocytes. Fibrosis refers to the type and degree of fibrosis in the liver. After long-term FFD, WT and Slc-HKO livers (fibrosis score of 1) displayed mild portal or periportal fibrosis. n = 4 to 6 (B). Scale bars = 100 μm (A).