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. 2022 Sep;192(9):1259–1281. doi: 10.1016/j.ajpath.2022.06.002

Figure 2.

Figure 2

Gene targeting disrupts Slc25a34 expression in the liver. A: Schematic depicting wild-type (WT) Slc25a34 gene structure, coding sequence, and protein domains. B: Simplified schematic of the floxed Slc25a34 allele (loxP sites are highlighted) and knockout allele after Cre-mediated recombination. A detailed scheme is shown in Supplemental Figure S2. C: Slc25a34 mRNA expression in livers from whole body knockouts (Slc-KO), normalized to Gapdh. D: Slc25a34 protein expression in mitochondrial fractions from WT control, Slc-KO, and Mir122-KO mice. Representative western blot is shown (left), and relative expression normalized to TOMM20 and quantified by densitometry (right). E: Slc25a34 mRNA expression in livers from hepatocyte-specific Slc25a34 knockout (Slc-HKO) mice, normalized to 18S. F: Slc25a34 protein expression in mitochondrial fractions from WT control and Slc-HKO mice. Representative western blot is shown (left), and relative expression normalized to TOMM20 and quantified by densitometry (right). Graphs represent means ± SEM. n = 3 (C and D); n = 4 (E); n = 3 to 5 (F). ∗P < 0.05, ∗∗P < 0.01.