Table 2.

Primers and TaqMan Probes (Both from Thermo Fisher Scientific) for Gene Expression, mtDNA Copy Number, and Genotyping Assays

Application	Gene/locus	Name	Sequence or TaqMan identifier
Gene expression	18S Rrna	18S-fwd	5′-TGACGGAAGGGCACCACCAG-3′
	18S Rrna	18S-rev	5′-TCGCTCCACCAACTAAGAACGGC
	Acly	Acly-fwd	5′-GGAGTCAAATCCTGGCTAAAACCTC-3′
	Acly	Acly-rev	5′-TTTCGTCCACACCCACAAGCAG-3′
	B2m	B2M-fwd	5′-TTCAGTATGTTCGGCTTCCC-3′
	B2m	B2m-rev	5′-CCTGGTCTTTCTGGTGCTTG-3′
	Cpt1a	Cpt1a-fwd	5′-CTCAGTGGGAGCGACTCTTCA-3′
	Cpt1a	Cpt1a-rev	5′-GGCCTCTGTGGTACACGACAA-3′
	Gapdh	Gapdh-fwd	5′-AAGGTCGGTGTGAACGGATTTGG-3′
	Gapdh	Gapdh-rev	5′-CGTTGAATTTGCCGTGAGTGGAG-3′
	Ppargc1a	Ppargc1a-fwd	5′-GTCATTCGGGAGCTGGATGG-3′
	Ppargc1a	Ppargc1a-rev	5′-CAACCAGAGCAGCACACTCT-3′
	Ppargc1b	Ppargc1b-fwd	5′-CACAAAGCGACTCTCTCTTGGCA-3′
	Ppargc1b	Ppargc1b-rev	5′-GGCTTGTATGGAGGTGTGTGGT-3′
	Pprc	PRC-fwd	5′-TCGAGTCTGTTGGAGCAGTTT-3′
	Pprc	PRC-rev	5′-GGGAATGTCAATGCCTGAGTTT-3′
	Srebf1	Srebf1-fwd	5′-TGGTTGTTGATGAGCTGGAG-3′
	Srebf1	Srebf1-rev	5′-AAAGACAAGGGGCTACTCTGGGAG-3′
	Tfam	Tfam-fwd	5′-GCCCGAGCGATGGCG-3′
	Tfam	Tfam-rev	5′-CAGACAAGACTGATAGACGAGGG-3′
	18S Rrna	TaqMan Probe	ID# Mm03928990_m1
	Slc25a34	TaqMan Probe	ID# Mm01350550_m1
mtDNA copy number	Mt-Co1	TaqMan Probe	ID# Mm04225243_g1
	Tfrc	TaqMan Probe	ID# 4458366
Genotyping	Slc25a34	Slc25a34-F32	5′-CTGCTGGCCTTCTCTACCAG-3′
	Slc25a34	Slc25a34-F51	5′-CCCTCCCTTGCATTCACTCC-3′
	Slc25a34	Slc25a34-R32	5′-TTTGGGACTGTGTTGCCACT-3′
	Slc25a34	Slc25a34-R51	5′-GTCTTGGAACTCCACCTGCT-3′
	Slc25a34	Slc25a34-R53	5′-AAAGCCCTAGGCTTGTGGTG-3′

mtDNA, mitochondrial DNA.