Table 4.
Differential Activation of Upstream Regulators by Slc-HKO Livers Compared with WT Controls (Short-term FFD)
| Upstream regulator | Predicted activation state | Activation Z score | P value of overlap |
|---|---|---|---|
| ITPR2 | Inhibited | −2.3 | 1.70 × 10−10 |
| PNPT1 | Activated | 3.5 | 5.33 × 10−10 |
| IRF7 | Inhibited | −4.9 | 1.98 × 10−09 |
| SIRT1 | Activated | 4.0 | 3.14 × 10−09 |
| XBP1 | Inhibited | −3.4 | 8.83 × 1−09 |
| NKX2-3 | Activated | 3.1 | 1.41 × 10−08 |
| PIK3CG | Activated | 2.9 | 1.81 × 10−08 |
| IGRM1 | Activated | 4.3 | 1.04 × 10−07 |
| TRIM24 | Activated | 4.4 | 2.76 × 10−07 |
| G protein alpha | Inhibited | −3.5 | 3.07 × 10−07 |
| miR-21 | Activated | 2.6 | 6.01 × 10−07 |
| STAT1 | Inhibited | −4.3 | 6.89 × 10−07 |
| ZC3H12C | Activated | 2.5 | 1.24 × 10−06 |
| IFNAR | Inhibited | −4.0 | 2.12 × 10−06 |
| IRF3 | Inhibited | −4.5 | 4.42 × 10−06 |
| G protein alpha | Inhibited | −2.5 | 8.21 × 10−06 |
| IFNL1 | Inhibited | −3.8 | 1.01 × 10−05 |
| NR1I3 | Activated | 2.9 | 1.25 × 10−05 |
| PTGER4 | Activated | 3.3 | 1.33 × 10−05 |
| ACKR2 | Activated | 3.2 | 2.29 × 10−05 |
A total of 105 Upstream Regulators were identified (Z score less than −2 and >2; P value of overlap <0.05), and the top 20 are shown. Bold indicates metabolic relevance.
FFD, fast-food diet; Slc-HKO, hepatocyte-specific Slc25a34 knockout; WT, wild type.