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. 2022 Aug 17;8(9):3969–3976. doi: 10.1021/acsbiomaterials.2c00330

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© 2022 The Authors. Published by American Chemical Society

Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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Cell viability after 9 days of IVD culture under physiological conditions in the multiaxial bioreactor. The viability in the 6 DOF model was assessed with lactate dehydrogenase and ethidium homodimer staining and quantified in outer AF, inner AF, and NP regions on IVD sections. Day 0 represents a nonloaded positive control group. Data are shown as the mean of 4 (outer AF) or 6 (inner AF and NP) regions of interest from 4 biological replicates + standard deviation. Statistical analysis was performed with an unpaired nonparametric t-test, where p < 0.001 (***) was statistically significant.