KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| C13orf8/CHAMP1 | Abnova | Cat# H00283489-B01P; RRID:AB_1673079 |
| Mad2L2/REV7 | Abcam | Cat# ab180579; RRID:AB_2890174 |
| POGZ | Bethyl Laboratories | Cat# A302-509A; RRID:AB_1966082 |
| TRIP13 | Abcam | Cat# ab128171; RRID:AB_11145670 |
| H3 | Cell Signaling Technology | Cat# 3638; RRID:AB_1642229 |
| GFP | Cell Signaling Technology | Cat# 2956; RRID:AB_1196615 |
| β-Actin | Cell Signaling Technology | Cat# 3700; RRID:AB_2242334 |
| RPA2-P-Ser33 | Fisher Scientific | Cat# NB100544; RRID:AB_526631 |
| RAD51 | Santa Cruz | Cat# sc-8349; RRID:AB_2253533 |
| GAPDH | Cell Signaling Technology | Cat# 5174; RRID:AB_10622025 |
| Goat anti-Rat IgG (Alexa Fluor 488) | Invitrogen | Cat# A-11006; RRID:AB_2534074 |
| Goat anti-Mouse IgG (Alexa Fluor 488) | Invitrogen | Cat# A32723; RRID:AB_2633275 |
| Chicken anti-mouse IgG (Alexa Fluor 594) Anti-rabbit IgG, HRP-linked | Invitrogen | Cat# A-21201; RRID:AB_2535787 |
| Flag | Millipore-Sigma | F3165; RRID:AB_259529 |
| Chemicals, peptides, and recombinant proteins | ||
| Olaparib | Selleckchem | S1060 |
| BrdU | Millipore-Sigma | B5002 |
| Anti-FLAG® M2 Magnetic Beads | Millipore-Sigma | M8823 |
| GFP-Trap_A | Chromotek | Gta-20 |
| Critical commercial assays | ||
| CellTiter-Glo Luminescent Viability Assay | Promega | G7570 |
| Subcellular protein fractionation kit | ThermoFisher | 78840 |
| Deposited data | ||
| Raw imaging data | This paper; Mendeley data | https://doi.org/10.17632/fkh6v4dw6y.1 |
| TCGA Pan-Cancer Atlas Studies’ RNASeq expression data | TCGA (PMID: 24071849) | https://www.cbioportal.org/ |
| Cancer cell lines’ RNASeq expression data | Cancer Cell Line Encyclopedia (Ghandi et al., 2019) | https://depmap.org/portal/ |
| Cancer cell lines’ dependency data (CRISPR DepMap Public 19Q4 dataset) | Broad Institute Cancer Dependency Map (Meyers et al., 2017) | https://depmap.org/portal/ |
| Experimental models: Cell lines | ||
| 293T | ATCC | N/A |
| U2OS | ATCC | N/A |
| RPE-1 | ATCC | N/A |
| HCC1937 | D’Andrea lab | N/A |
| U2OS-DR-GFP | D’Andrea lab | N/A |
| U2OS-EJ5 | D’Andrea lab | N/A |
| Oligonucleotides | ||
| siCHAMP1-4: CAGGAATCAAGCAGTGATCAA | Qiagen | SI00318038 |
| siCHAMP1-8: CAGGAACTTCGTAAACCATCA | Qiagen | SI04282159 |
| SiPOGZ: CCGGGTGTAAGGTCCCTTTAA | Qiagen | SI00688583 |
| siTRIP13: TTGGGACAGCTTGGTATACGA | Qiagen | (Clairmont et al., 2020) |
| AllStars Neg. Control siRNA | Qiagen | 0001027281 |
| Recombinant DNA | ||
| CHAMP1 CRISPR guide RNA 1_pSpCas9 BB-2A-GFP (PX458) | Genscript | N/A |
| CHAMP1 CRISPR guide RNA 3_pSpCas9 BB-2A-GFP (PX458) | Genscript | N/A |
| POGZ CRISPR guide RNA 1_pSpCas9 BB-2A-GFP (PX458) | Genscript | N/A |
| POGZ CRISPR guide RNA 2_pSpCas9 BB-2A-GFP (PX458) | Genscript | N/A |
| Software and algorithms | ||
| ImageJ | U. S. National Institutes of Health | https://imagej.nih.gov/ij/ |
| Graphpad Prism 9 | Graphpad | https://www.graphpad.com/ |
| R (version 4.0.3) | R Foundation for Statistical Computing | https://www.R-project.org/ |
| R package survival (version 3.2–10) | R Foundation for Statistical Computing | https://CRAN.R-project.org/package=survival |
| R package survminer (version 0.4.9) | R Foundation for Statistical Computing | https://CRAN.R-project.org/package=survminer |
| R package edgeR (version 3.32.1) | Bioconductor | https://www.bioconductor.org/packages/release/bioc/html/edgeR.html |
| R package ggplot2 (version 3.3.5) | R Foundation for Statistical Computing | https://ggplot2.tidyverse.org |
| R package ggpubr (version 0.4.0) | R Foundation for Statistical Computing | https://CRAN.R-project.org/package=ggpubr |
| R package ggpmisc (version 0.4.3) | R Foundation for Statistical Computing | https://CRAN.R-project.org/package=ggpmisc |