TABLE 1.
B. subtilis strains used
| Strain | Genotype | Source or reference |
|---|---|---|
| SMY | Wild type | P. Schaeffer |
| SMY-S | ΔgltC::spc | 2 |
| PY150 | gltC150::(Tn917 erm) | P. Youngman |
| SX150103 | gltC150::(Tn917 erm) gltAp3 | This work |
| SF62 | tnrA62::(Tn917 erm) trpC2 | 40 |
| SF73 | glnA73 | 14 |
| BB278 | tnrA62::(Tn917 erm) | SMY × SF62 DNA |
| SF73-T | glnA73 tnrA62::(Tn917 erm) | SF73 × BB278 DNA |
| BB614 | ΔgltC::spc gltR24 ΔamyE::[cat Φ(gltC′p+-gusA) Φ(gltA′p+-lacZ)] | 3 |
| BB873 | ΔgltC::spc gltR24 tnrA62::(Tn917 erm) ΔamyE::[cat Φ(gltC′p+-gusA) Φ(gltA′p+-lacZ)] | BB614 × BB278 DNA |
| LG200 | ΔamyE::[cat Φ(gltC′p+-gusA) Φ(gltA′p+-lacZ)] | SMY × pLG200 (2) |
| LG200-S | ΔgltC::spc ΔamyE::[cat Φ(gltC′p+-gusA) Φ(gltA′p+-lacZ)] | 2 |
| LG203 | ΔamyE::[cat Φ(gltC′p3-gusA) Φ(gltA′p3-lacZ)] | SMY × pLG203 |
| LG203-S | ΔgltC::spc ΔamyE::[cat Φ(gltC′p3-gusA) Φ(gltA′p3-lacZ)] | SMY-S × LG203 DNA |
| LG203-T | tnrA62::(Tn917 erm) ΔamyE::[cat Φ(gltC′p3-gusA) Φ(gltA′p3-lacZ)] | BB278 × LG203 DNA |
| LG203-ST | ΔgltC::spc tnrA62::(Tn917 erm) ΔamyE::[cat Φ(gltC′p3-gusA) Φ(gltA′p3-lacZ)] | LG203-S × BB278 DNA |
| LG219 | ΔamyE::[cat Φ(gltC′p19-gusA) Φ(gltA′p19-lacZ)] | SMY × pLG219 |
| LG219-S | ΔgltC::spc ΔamyE::[cat Φ(gltC′p19-gusA) Φ(gltA′p19-lacZ)] | LG219 × SMY-S DNA |
| LG219-T | tnrA62::(Tn917 erm) ΔamyE::[cat Φ(gltC′p19-gusA) Φ(gltA′p19-lacZ)] | LG219 × SF62 DNA |
| LG219-ST | ΔgltC::spc tnrA62::(Tn917 erm) ΔamyE::[cat Φ(gltC′p19-gusA) Φ(gltA′p19-lacZ)] | LG219-S × BB278 DNA |
| LG219/24 | ΔamyE::[cat Φ(gltC′p19/24-gusA) Φ(gltA′p19/24-lacZ)] | SMY × pLG219/24 |
| LG219/24-T | tnrA62::(Tn917 erm) ΔamyE::[cat Φ(gltC′p19/24-gusA) Φ(gltA′p19/24-lacZ)] | LG219/24 × BB278 DNA |
| BB1045 | gltC150::(Tn917 erm) gltAp3 ΔamyE::[cat Φ(gltC′p19-gusA) Φ(gltA′p19-lacZ)] | SX150103 × LG219 DNA |
| BB1068 | tnrA62::(Tn917 erm::spc) | BB278 × p917::spc (36a) |
| BB1082 | gltC150::(Tn917 erm) gltAp3 ΔamyE::[cat Φ(gltC′p3-gusA) Φ(gltA′p3-lacZ)] | SX150103 × LG203 DNA |
| BB1083 | gltC150::(Tn917 erm) tnrA62::(Tn917 erm)::spc ΔamyE::[cat Φ(gltC′p3-gusA) Φ(gltA′p3-lacZ)] | BB1082 × BB1068 DNA |
| BB1194 | glnA73 ΔamyE::[cat Φ(gltC′p19-gusA) Φ(gltA′p19-lacZ)] | SF73 × LG219 DNA |
| BB1195 | glnA73 tnrA62::(Tn917 erm) ΔamyE::[cat Φ(gltC′p19-gusA) Φ(gltA′p19-lacZ)] | SF73-T × LG219 DNA |
| BB1199 | glnA73 ΔamyE::[cat Φ(gltC′p19/24-gusA) Φ(gltA′p19/24-lacZ)] | SF73 × LG219/24 DNA |
| LG700 | ΔamyE::[cat Φ(gltC′p700-gusA) Φ(gltA′p700-lacZ)] | 2 |
| LG700-S | ΔgltC::spc ΔamyE::[cat Φ(gltC′p700-gusA) Φ(gltA′p700-lacZ)] | SMY-S × LG700 DNA |
| LG700-ST | ΔgltC::spc tnrA62::(Tn917 erm) ΔamyE::[cat Δ(gltC′p700-gusA) Φ(gltA′p700-lacZ)] | LG700-S × BB278 DNA |
| LG702 | ΔamyE::[cat Φ(gltC′p702-gusA) Φ(gltA′p702-lacZ)] | 2 |
| LG702-S | ΔgltC::spc ΔamyE::[cat Φ(gltC′p702-gusA) Φ(gltA′p702-lacZ)] | SMY-S × LG702 DNA |
| LG702-ST | ΔgltC::spc tnrA62::(Tn917 erm) ΔamyE::[cat Φ(gltC′p702-gusA) Φ(gltA′p702-lacZ)] | LG702-S × BB278 DNA |
| LG708 | ΔamyE::[cat Φ(gltC′p708-lacZ) Φ(gltA′p708-gusA)] | SMY × pLG708 |
| LG711 | ΔamyE::[cat Φ(gltC′p711-lacZ) Φ(gltA′p711-gusA)] | SMY × pLG711 |