TABLE 1.
Catalog numbers and dilution factors of the antibodies used in this study
| Antibody | Catalog no. | Clone name | Dilution factor | Manufacturer |
|---|---|---|---|---|
| Mouse anti-p17a | 2,000 | Our laboratory | ||
| Mouse anti-p53 | 2527 | 7F5 | 3,000 | Cell Signaling |
| Rabbit anti-p-p53 (S15)a | 9284 | 2,000 | Cell Signaling | |
| Rabbit anti-PTEN | 9559 | 138G6 | 3,000 | Cell Signaling |
| Rabbit anti-p-PTEN (S380/T382/383) | 9554 | 2,000 | Cell Signaling | |
| Rabbit anti-Akt | 4691 | C67E7 | 3,000 | Cell Signaling |
| Rabbit anti-p-Akt (T308) | 2965 | C31E5 | 2,000 | Cell Signaling |
| Rabbit anti-p-Akt (S473) | 3787 | 736E11 | 2,000 | Cell Signaling |
| Rabbit anti-TSC2 | 3990 | D57A9 | 2,000 | Cell Signaling |
| Rabbit anti-p-TSC2 (S939) | 3615 | 1,000 | Cell Signaling | |
| Rabbit anti-p-TSC2 (T1462) | 3617 | 5B12 | 1,000 | Cell Signaling |
| Rabbit anti-PRAS40 | 2691 | D23C7 | 3,000 | Cell Signaling |
| Rabbit anti-p-PRAS40 (T246) | 13175 | D4D2 | 3,000 | Cell Signaling |
| Mouse anti-Rheb | sc-271509 | B-12 | 1,000 | Santa Cruz |
| Rabbit anti-FKBP38 | sc-66894 | H-220 | 1,000 | Santa Cruz |
| Rabbit anti-raptor | 2280 | 24C12 | 3,000 | Cell Signaling |
| Rabbit anti-p-raptor (S863) | sc-130214 | 1,000 | Santa Cruz | |
| Mouse anti-FKBP12 | sc-100282 | L-17 | 1,000 | Santa Cruz |
| Rabbit anti-mTOR | 2983 | 7C10 | 3,000 | Cell Signaling |
| Mouse anti-p-mTOR (S2448)a | 2971 | 2,000 | Cell Signaling | |
| Rabbit anti-LAMP1 | 9091 | D2D11 | 3,000 | Cell Signaling |
| Rabbit anti-eIF4Ea | 3591 | 2,000 | Cell Signaling | |
| Rabbit anti-p-eIF4E (S209) | 9741 | 2,000 | Cell Signaling | |
| Rabbit anti-p70 S6ka | 9202 | 3,000 | Cell Signaling | |
| Mouse anti-p-p70 S6k (T389) | 9234 | 108D2 | 1,000 | Cell Signaling |
| Rabbit anti-ULK1a | 4773 | R600 | 3,000 | Cell Signaling |
| Rabbit anti-p-ULK1 (S757)a | 6888 | 2,000 | Cell Signaling | |
| Mouse anti-Beclin 1 | 3495 | D40C5 | 3,000 | Cell Signaling |
| Rabbit anti-PI3k class III | 4263 | D9A5 | 2,000 | Cell Signaling |
| Rabbit anti-4EBP1 | 9644 | 53H11 | 2,000 | Cell Signaling |
| Rabbit anti-LC3Ba | 2775 | 2,000 | Cell Signaling | |
| Mouse anti-β-actin | MAB1501 | C4 | 10,000 | Millipore |
| Goat anti-mouse IgG (H + L) HRP | 5220-0341 | 5,000 | SeraCare | |
| Goat anti-rabbit IgG (H + L) HRP | 5220-0336 | 5,000 | SeraCare |
a
Polyclonal antibody.