FIG 5.
Entry and immune escape of SARS-CoV-2 variants. (A) The entries of SARS-CoV-2 D614, G614, G614-Y453F, G614-F486L, and G614-N501T pseudoviruses into huh7 cells were evidenced by cellular GFP expression. The representative results from three experiments are shown. Relative infectivity was normalized against that of the G614 pseudovirus. Statistical significance was analyzed using a one-way analysis of variance with Tukey’s multiple comparison test for multiple groups. (B) Antibody proteins in the supernatant were captured in the protein A chip. Serially diluted SARS-CoV-2 WT RBD, RBD Y453F, RBD F486L, and RBD N501T proteins were used to evaluate binding affinities to antibodies. KD values are means ± SEM of three independent replicates (n = 3). The representative results from three experiments are shown. (C) The pVNT50 against SARS-CoV-2 G614, G614-Y453F, G614-F486L, and G614-N501T pseudoviruses in sera from five volunteers who received three doses of ZF2001 vaccine. The horizontal bars show the geometric mean titer line with 95% confidential interval. Two-tailed Wilcoxon matched-pairs signed-rank test was used to analyze the neutralization values between different variants. The pVNT50 for each sample was determined twice.