FIG 1.

Maximum-likelihood phylogenetic tree of the NS5 (RNA-dependent RNA polymerase) amino acid sequences of flaviviruses, trimmed with BMGE v1.2 (66), under an LG+F+R8 protein substitution model in IQ-TREE v1.6.12 (67). Aquatic host-linked flaviviruses are highlighted in blue—light blue for freshwater hosts or dark blue for marine hosts. A, B, and C refer to clades that have not yet been tentatively named. The Tree file, alignment, and table containing accession numbers of used sequences are available on GitHub (https://doi.org/10.5281/zenodo.6980038).