FIG 7.
Experimental scheme and mortality and virus quantification in suckling mice after SADS-CoV infection. (A) Female mice were intragastric inoculated with 1.5 × 106 TCID50 SADS-CoV or an equal volume of DMEM (mock control) three times (left) and mated. Two-day-old suckling mice were intragastrically inoculated with 8 × 105 TCID50 SADS-CoV and were observed for clinical signs daily for 14 days (right). Survival rate (B) and viral loads (C) in the heart, lungs, liver, spleen, kidneys, stomach, small intestine, large intestine, and brain, collected from 2-day-old suckling mice at the end point of the trial. *, P < 0.05; ***, P < 0.0001.