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. 2022 Aug 16;96(17):e00707-22. doi: 10.1128/jvi.00707-22

FIG 5.

FIG 5

Trim7 binds NS6 but not NS7 nor the NS6-7 precursor protein. (A) Western blot from coimmunoprecipitation experiment conducted in HEK 293T cells cotransfected with Ty1-tagged Trim7 (T7) or control Trim protein (C) with Flag-tagged GFP, NS6, or NS7 (n = 3). (B) Coomassie brilliant blue stain of a representative glutathione S-transferase (GST) pulldown assay with recombinant GST- or MBP-tagged proteins purified from E. coli. The GST-NS67 protein represents a fusion of NS6-7 that lacks autoprocessing due to mutations in the NS6 protease catalytic site. The B30.2 domain of Trim7 (MBP-B30.2) binds NS6 but not NS7 or NS6-7 (n = 4).