FIG 6.
Effect of HIV-1AD8 Env changes on BNM-III-170-induced exposure of ADCC epitopes. Activated primary CD4+ T cells were infected with the molecularly cloned viruses expressing the indicated Env variants. Two days after infection, cells were incubated with BNM-III-170 (50 μM) or DMSO and stained with anti-CD4, anti-BST-2 or 17b, 2G12, and A32 antibodies or HIV+ plasma followed by detection of intracellular p24. (A and B) The binding of 17b (A) or HIV+ plasma (B) relative to the binding of 2G12 is shown. Similar results were obtained with the A32 antibody (not shown). (C and D) The mean fluorescence intensity (MFI) for the staining of cells with antibodies against CD4 (C) and BST-2 (D) is shown. Means and standard deviations from two independent experiments are shown.