TABLE 2.
Affinity of monomeric gp120 from the different Env variants for sCD4a
| gp120 | KD (nM) | ka (1/Ms) | kdis (1/s) |
|---|---|---|---|
| AD8-WT | 13.46 ± 0.05 | 5E+04 ± 5E+03 | 7E-04 ± 7E-05 |
| E64G | 11.04 ± 1.93 | 7E+04 ± 2E+04 | 7E-04 ± 7E-05 |
| S375N | 17.85 ± 3.63 | 1E+05 ± 2E+03 | 2E-03 ± 3E-04 |
| I424T | 4.04 ± 0.02 | 6E+04 ± 3E+03 | 2E-04 ± 1E-05 |
| E64G + S375N | 8.83 ± 3.89 | 7E+04 ± 3E+04 | 6E-04 ± 3E-05 |
| E64G + I424T | 17.73 ± 6.65 | 1E+05 ± 8E+03 | 2E-03 ± 9E-04 |
| S375N + I424T | 25.51 ± 11.10 | 8E+04 ± 2E+04 | 2E-03 ± 3E-04 |
| E64G + S375N + I424T | 48.54 ± 1.56 | 6E+04 ± 5E+03 | 3E-03 ± 3E-04 |
a
The means and standard deviations derived from two independent experiments are shown. Association of sCD4 with monomeric gp120 from the different variants was estimated by biolayer interferometry, with the calculated on-rates (ka), off-rates (kdis), and equilibrium dissociation constants (KD). E represents 10 raised to the given exponential.