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Ocular swabs from HSV-1 infected mice. Eight-week-old C57BL/6 mice were ocularly infected with ~2 × 105 PFU either HSV-1 strain McKrae or dLAT2903 without scarification as previously described (34). Ocular swabs were obtained every other day for 10 days and then every 5 days for a total of 30 days postinfection (n = 5 mice/group). Samples were freeze-thawed three times prior to plaquing on Vero cells to measure titers of infectious virus.
