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. 2022 Sep 14;130(9):097003. doi: 10.1289/EHP10923

Figure 1.

Figure 1 is a timeline in the form of an arrow pointing toward the right, depicting five stages of study, including Baseline, Initial Dosing, Breeding and Pregnancy, Postpartum, and Necropsy. The blood samples for complete blood counts plus serum chemistry were collected for Baseline and Initial Dosing. The levels of cytokines and chemokines in the blood were analyzed at Baseline and Initial Dosing for an average of 43 days. The breeding was between 1 and 8 months, and the pregnancy, six months. The magnetic resonance imaging was conducted during the Postpartum period. The levels of cytokines and chemokines in the blood were analyzed, and histopathology and ribonucleic acid sequencing were conducted during Necropsy. There was a three times a week behavior assessment, with weekly weights, and a biweekly exposure assessment.

Timeline of study. Representation of the five stages of the study: Baseline (prior to dosing), Initial Dosing (∼2 months prior to breeding), Breeding and Pregnancy, Postpartum. Necropsy occurred in the Postpartum period. Daily, oral domoic acid (DA) dosing began in the Initial Dosing period and continued through Breeding and Pregnancy. A subset of animals was continued on daily dosing in the Postpartum period (see Table S1 for details). Blood samples for complete blood counts (CBCs) and serum chemistry were collected at the beginning of study (Baseline) and at the end of the Initial Dosing period (average: day 73). Blood levels of cytokines and chemokines were analyzed twice during Baseline, twice during Initial Dosing (average: day 15, day 43), and at necropsy (average: month 14 or 425 d). Magnetic resonance (MR) imaging was conducted during the Postpartum period. Tissue was collected at necropsy for histological assessment and RNA sequencing (RNAseq). Body weights, behavioral assessments, and DA exposure assessments were collected throughout the entire study.