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. 2022 Sep 14;12:15451. doi: 10.1038/s41598-022-18906-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Embryo Health is Negatively Affected by SARS-CoV-2 Infection. Sample images of infected embryos with various degrees of cytopathic effects. (A,B) Embryo with cell damage (yellow arrowheads) in fluorescent (infected) cells in the herniating and zona pellucida compartments. (C) Infected herniating embryo (bottom) with severely affected health, and non-infected embryo (top) with good health. (D) Hatched infected embryo next to empty zona pellucida, displaying global cell death. (E,F) Examples of infected embryos displaying total collapse and death. (G) Summary of embryo health 12–16 h after exposure to SARS-CoV-2. Top bar includes all embryos with evidence of infection (fluorescent cells). Bottom bar includes all embryos with no evidence of infection (no fluorescent cells). Scale bars represent 50 µm.