Neuronal marker positivity in the middle frontal and inferior temporal gyrus of human post-mortem brains with common tauopathies. Low and high magnification images showing multiplex immunofluorescence staining of neurons (NeuN, blue), active caspase-6 (aCasp-6, orange), neoepitope mAbs of caspase-6-cleaved tau sites (D402 and D13, green and cyan, respectively), and phosphorylated tau (Ser 202; p-tau, magenta). Compared to other tauopathies (B–E), AD (A) showed the most robust positivity for all markers, while no or negligible positivity was observed in controls (F). Abbreviations: AD, Alzheimers disease (A); PiD, picks disease (B); CBD, corticobasal degeneration (C); PSP progressive supranuclear palsy (D); AGD, argyrophilic grain disease. Scale bars: 50 μm