TABLE 1.
Bacterial strains, bacteriophages, and plasmids
| Strain, phage, or plasmid | Relevant feature(s) | Source or reference |
|---|---|---|
| Strains | ||
| JC1569 | F′::Tn10lac+Ts TcrmetB1 leu-6 his-1 arg-6 rec acY1 xyl-7 mtl-2 gal-6 str-104 tonA2 tsx-1λ− sup-E44 | J. Ou |
| HER1266′ | Mutant (Lac−) of O157:H7 (HER1266); AR1 sensitive | This study |
| TM | Tn10 mutant of HER1266′; AR1 resistant | This study |
| JM109 | F′ traD36 lacIq Δ(lacZ)M15 proA+B+/e14− (McrA−) Δ(lac-proAB)thi gyrA96 (nNalr) endA1 hsdR17(rK− mK+) relA1 supE44 recA1 | Stratagene |
| KM | Tn10 mutant of JM109; T4 resistant | This study |
| Bacteriophages | ||
| AR1 | O157:H7-specific coliphage | 34 |
| T4 | Coliphage | ATCC 11303-B4 |
| Plasmids | ||
| pBluescript II SK(+) | Cloning vector (Ampr) | Stratagene |
| pGex-2T | Expression vector (Ampr) | 38 |
| pGexO157OmpC | Derived from pGex-2T; expresses GST-OmpC(O) | This study |
| pTac-85 | Expression vector (Ampr) | 19 |
| pTacOmpC(O) | Derived from pTac-85; expresses OmpC(O) | This study |
| pTacOmpC(K) | Derived from pTac-85; expresses OmpC(K) | This study |