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. 2022 Sep 6;479(17):1807–1824. doi: 10.1042/BCJ20210851

Figure 8. AhR mediates carbidopa-mediated IDO1 suppression in PDAC cells.

Figure 8.

(A) Real-time PCR showing relative mRNA expression of CYP1A1 in BxPC-3 cells treated with various concentrations of carbidopa (100 µM, 500 µM and 1000 µM) for 24 h. (B and C) Real-time PCR showing relative mRNA expression of IDO1 and CYP1A1 in BxPC-3 cells cultured with 10 nM TCDD, either in the presence or absence of an AhR antagonist, CH-223191. (D and E) Real-time PCR showing relative mRNA expression of IDO1 and CYP1A1 in HPAF-II cells cultured with 50 µM carbidopa, either in the presence or absence of an AhR antagonist, GNF351. (F) ChIP assay showing AhR binding to the IDO1 promoter. For all in vitro studies n = 3. Data are means ± SEM. Statistical significance were assessed using one-way ANOVA with Dunnett's post hoc test for (A) and Tukey's post hoc test for (B–E) (* P < 0.05; ** P < 0.01; *** P < 0.001; ns > 0.05). ns; not significant.