TABLE 2.
N-terminal sequence of membrane-associated proteins showing increased levels at pH 10.5
| Spot no. | N-terminal sequencea | Database match | Function or comments |
|---|---|---|---|
| 17 | APADAKFSDVSSxH(V)xID | None | Subsequent cloning of a gene fragment identified this spot as a surface layer protein |
| 33 | SNTAERVVKGGSFLVEDIE | B. subtilis YusJ | Similar to butyryl-CoA dehydrogenase |
| 36 | APADAKFSDVSS(S)H(W)SI | Spot 17 | Degradation product of spot 17? |
| 41 | ATEITMPQLGESVTEG(T)I | B. subtilis BfmBB | Branched-chain α-keto acid dehydrogenase |
| 73 | APADAKFSDVxx(N)(H)(E) | Spot 17 | Degradation product of spot 17? |
| 100 | MNFDLTKEQKMIRDMVRD | B. subtilis YngJ | Similar to butyryl-CoA dehydrogenase |
| 121 | AVISYIEAVTLALKEEM | B. subtilis BfmBAB | Branched-chain α-keto acid dehydrogenase |
a
x, unknown; probable residues are shown in parentheses.