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Primer extension analysis of the ccpA promoter. The transcriptional start site is indicated with an arrow. The −10 region in the coding strand is boxed. RNA was isolated from S. thermophilus CNRZ302 grown on glucose (G) or lactose (L), and primer extension products were run parallel to a sequence ladder (lanes A, C, G, and T) obtained with the same primer. Approximately 15 μg of RNA was used per primer extension reaction.
