Fig. 10. (A) The viability of C. reinhardtii upon incubation at pH 5.5 with aqueous solutions of different concentrations of BRB encapsulated in ODTAB-coated BRB-loaded shellac NPs. The solutions were prepared from 0.05 wt% BRB (shellac) NPs stock solution coated with 0.05 wt% ODTAB. (B) The C. reinhardtii viability upon incubation with 0.0001 wt% encapsulated BRB in shellac NPs, 0.0001 wt% free BRB, 0.0005 wt% shellac NPs coated with 0.0001 wt% ODTAB, 0.0001 wt% BRB–NPs coated with 0.0001 wt% ODTAB, and 0.0001 wt% pure ODTAB at pH 5.5 and at room temperature. (C)–(F) SEM images of C. reinhardtii whereby (C) represent the control sample. (D)–(F) C. reinhardtii incubated with 0.005 wt% BRB loaded shellac NPs coated with 0.005 wt% ODTAB after 2 hours at room temperature.