The cytotoxicity effect of each component on C. reinhardtii, yeast, and E. coli which represented by (++++: very strong, +++: strong, ++: medium, and +: weak).

Nanocarrier component	Antimicrobial effect on
	C. reinhardtii	Yeast	E. coli
Shellac NPs stabilised with P407	+	+	+
Shellac NPs stabilised with P407 and coated ODTAB	+	+	+
Free BRB	+++	+	+++
Shellac NPs loaded with BRB and stabilised with P407	++	+	++
Shellac NPs loaded with BRB, stabilised with P407 and coated with ODTAB	++++	+++	++++