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. Author manuscript; available in PMC: 2023 Oct 1.
Published in final edited form as: Hypertension. 2022 Aug 22;79(10):2250–2261. doi: 10.1161/HYPERTENSIONAHA.122.19862

Figure 4. Role of H2O2 in phosphorylated eNOS from S1P.

Figure 4.

S1P-induced Ser1177 phosphorylation of eNOS in HUVECs (n=4) is impaired in the presence of PEG-Catalase (500U, n=4) and significantly reduced during inhibition of NOX4 with GKT137831 (1μM, n=4). Representative images of western blots on the left (A) and phospho-eNOS:eNOS ratio comparison on the right (B). Data are expressed as the mean ratio of phosphorylated eNOS to total eNOS±SEM. *p<0.05 compared to S1P alone.