. 2000 Nov;182(21):6014–6026. doi: 10.1128/jb.182.21.6014-6026.2000

TABLE 3.

Sensitivity of RK2 derivatives to IncC-mediated incompatibility

Test plasmid	Relevant properties	Induced plasmid	Relative EOP^a	Incompatibility^b
pRK2013	RK2ΔkilA ΔkilE ΔkilC Δpar	Vector	1.3	−
	Eight O_B sites	tacp-incC2⁺	2.4 × 10⁻⁵	+
pRK21261	incC⁺ korABFG⁺ kfrA⁺ upf54.8⁺ O_B1⁺ O_B2⁺ O_B3⁺	Vector	1.0	−
		tacp-incC2⁺	2.2 × 10⁻⁵	+
pRK2362	korA⁺ incC⁺ korB⁺ O_B1⁺	Vector	0.8	−
		tacp-incC2⁺	1.0 × 1.0⁻³	+
pRK2366	korA⁺ incC⁺ korB′ O_B1⁺	Vector	1.0	−
		tacp-incC2⁺	0.7	−
pRK2178	korA⁺ incC⁺ korB⁺ korF⁺O_B1⁻	Vector	0.8	−
		tacp-incC2⁺	0.9	−

EKA335 was the host strain. Cells were plated on medium selecting both plasmids and containing or lacking IPTG. The relative EOP was calculated as follows: CFU on medium with IPTG/CFU on medium without IPTG.

−, compatible; +, incompatible.