Skip to main content
. 2022 Sep 14;8(37):eabn5732. doi: 10.1126/sciadv.abn5732

Fig. 7. NLRC5 KD in H1-derived islet-like cells decreases the T cell activation in coculture experiments.

Fig. 7.

H1-derived islet-like cells were transfected with an siRNA control (siCTL, white bars) or with an siRNA targeting NLRC5 (siNL#2, gray bars). Cells were then left untreated or treated with IFNα (2000 U/ml) for 24 hours. (A) NLRC5 protein expression was determined by Western blotting. NLRC5 protein was quantified by densitometry and normalized by the housekeeping protein β-actin and then by the highest value of each experiment considered as 1. NLRC5 depletion decreased the mRNA expression of HLA-ABC (B) and its cell surface expression, evaluated by flow cytometry (C). NLRC5 depletion decreased the mRNA expression of (D) HLA-E and (E) PDL1. mRNA expression was analyzed by RT-PCR and normalized by the geometric mean of GAPDH and β-actin and then by siCTL-treated cells exposed to IFNα and considered as 1. (F) T cell activation determined by the percentage of CD107a+ CD8+ T cells. Results are means ± SEM of four to seven independent experiments. ##P < 0.01, ###P < 0.001, and ####P < 0.0001 versus untreated and transfected with the same siRNA; *P < 0.05, ***P < 0.001, and ****P < 0.0001, as indicated by bars (ANOVA followed by Bonferroni correction for multiple comparisons).