TABLE 4.

Time-resolved Fluorescence anisotropy parameters.

System	θ1/ns	θ2/ns	θ3/ns
ANS in CHT*	0.056 (43)	0.712 (45)	30 (12)
ANS in CHT+1% formalin	0.098 (52)	0.584 (39)	30 (9)
ANS in CHT+2% formalin	0.094 (56)	0.425 (38)	30 (6)
ANS in CHT+4% formalin	0.092 (62)	0.244 (36)	30 (2)

^*

The anisotropy decay of ANS, in CHT, is fitted by the following equation.

$$\mathit{r}\left(\mathit{t}\right)={\mathit{r}}_0\left({\mathit{\beta }}_1\mathbf{exp}\left(-\frac{\mathit{t}}{{\mathit{\theta }}_1}\right)+{\mathbf{\beta }}_2\mathbf{exp}\left(-\frac{\mathit{t}}{{\mathit{\theta }}_2}\right)+{\mathbf{\beta }}_3\mathbf{exp}\left(-\frac{\mathit{t}}{{\mathit{\theta }}_3}\right)\right)$$

Where r₀ is initial anisotropy, β_is and θ_is are the amplitudes and corresponding rotational time constants, respectively. The longest rotational correlation time is kept constant at 30 ns. In presence of formalin, the anisotropy decays are fitted with Eq. 2 as described in the text.