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. 2022 Sep 13;11:e74955. doi: 10.7554/eLife.74955

Figure 4. The position of the proximal border of the progenitor zone does not correlate with the position of Sh1.

(A) Fluorescence confocal maximum projection of surface images (~7 µm depth) of fixed, dissected gonads showing the progenitor pool marked in magenta and the sheath cell marked in green. All measurements were made by examining optical sections through the entire depth of the gonad. Magenta caret represents the location of the proximal border of the CYE-1-positive, pSUN-1-negative progenitor pool (pSUN-1 staining not shown; see Materials and methods). Green caret represents the distal edge of Sh1. (B) Quantitative graph with magenta dots representing the proximal extent of the CYE-1 staining and green dots representing the distal reach of Sh1. Each pair of magenta and green dots connected by a line represents the data for a single gonad. (C) Dot plot with overlaid box plots showing the distance and quantiles of the progenitor pool and distal extent of Sh1 for each genotype. Each dot represents a single specimen of that genotype. p values were calculated using Student’s t-test. (D) Scatterplot showing lack of correlation between the proximal PZ border and Sh1 position. Scale bars are 10 µm. Strains used, top to bottom as indicated on figure panels A and C, and (n) number of gonads are DG5136 (72), DG5150 (26), DG5181 (52), DG5248 (30), and DG5249 (32). See Supplementary file 1 for complete genotypes.

Figure 4—source data 1. Source data for Figure 4 and Figure 4—figure supplement 1.

Figure 4.

Figure 4—figure supplement 1. The position of the proximal border of the progenitor zone does not correlate with the position of Sh1 when data are shown in cell diameters.

Figure 4—figure supplement 1.

(A) Quantitative graph with magenta dots representing the location of the proximal border of the CYE-1-positive, pSUN-1-negative progenitor pool and green dots representing the proximal extent of and green dots representing the distal reach of Sh1. Each pair of magenta and green dots connected by a line represents the data for a single gonad. (B) Dot plot with overlaid box plots showing the distance and quantiles of the CYE-1-positive progenitor pool and distal extent of Sh1 for each genotype, in cell diameters from the distal end. Each dot represents a single specimen of that genotype. p values were calculated using Student’s t-test. (C) Scatterplot showing lack of correlation between the proximal extent of progenitor zone and the distal reach of Sh1. CD: cell diameters. Strains used and n are listed in Figure 4.