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. 2022 Sep 1;11:e77775. doi: 10.7554/eLife.77775

Figure 6. Red-shifted oxidized MitoTimer signal is reduced in cav1.3a and otofb mutants.

Figure 6.

(A–C) Hair cells in wildtype (A), cav1.3a-/- mutant (B), and otofb-/- mutant (C) Tg[myosin6b:mitoTimer]w208 neuromasts at 5 dpf. (D–E) Average dot plots of the ratio of red/green MitoTimer fluorescence intensity at 3, 5, and 6 dpf in cav1.3a-/- mutant fish (blue) (D) and otofb-/- mutant fish (orange) (E) and respective siblings (black, gray) show that mutants exhibit reduced mitochondrial oxidation over time. Each dot in D and E represents one neuromast. A minimum of 3 animals and 10 neuromasts were examined per treatment group. Error bars: SEM. For comparisons, a two-way ANOVA with a Sidak’s correction for multiple comparisons was used in D-E. * p<0.05, *** p<0.001, **** p<0.0001. Scale bar = 5 µm.

Figure 6—source data 1. Mean numbers and statistics for Mitotimer labeling.