Figure 3. Dose-response screening data with selectivity and potency filters.

A) Number of compounds that qualify as hits for each DUB based on filter criteria listed in the table below. The arrow denotes increasing potency and/or selectivity of hit compounds for each DUB. The * denotes that for UCHL1, 25 μM was the highest tested dose in the dose-response and thus this value was used for calling hits as active against UCHL1.

B) The violin plot shows the distribution of IC₅₀s for DUBs that had qualifying hits in section IV in Fig 3A.