FIG. 2.

Phenotypes of constructs containing hybrids of adhE⁺ and adhE* on alcohol indicator medium. Red colonies indicate complete oxidation of ethanol to acetyl-CoA. Slanted hash marks represent fragments from wild-type adhE⁺; straight hash marks represent fragments from the adhE* mutant. Restriction sites used in the fragment exchange experiments are indicated. The adhE* mutations discussed in the text are boxed. Putative NAD and iron binding sites (Fe) are shown.