TABLE 1.
Specific activity of ADH clones
| Strain | Genotypea | Plasmidb | ADH activityc
|
|
|---|---|---|---|---|
| Aerobic | Anaerobic | |||
| CAS50 | DC271 (adhE+) | pACYC177 | <0.1 | 1.9 ± 0.53 |
| CAS51 | DC272 (adhC) | pACYC177 | 21.9 ± 0.1 | 20.1 ± 4.06 |
| CAS62 | Leo42 (ΔadhCE) | pACYC177 | <0.1 | ND |
| CAS56 | Leo42 (ΔadhCE) | pCRADH1 | 1.9 ± 0.5 | 7.8 ± 2.08 |
| CAS57 | Leo42 (ΔadhCE) | pCRADH2 | 9.3 ± 1.95 | 8.1 ± 1.84 |
a
Genotypes: DC271 is fadR mel tyrT (6), DC272 is fadR mel tyrT adhC (6), and Leo42 is fadR mel tyrT srl::Tn10 recA (ΔadhCE of DC272) (23).
b
Plasmids are described in the text and in Fig. 1.
c
ADH was assayed spectrophotometrically by monitoring the reduction of NAD+ to NADH at 340 nm (6). Activity is reported as nanomoles of NADH produced per minute per milligram of protein. ND, Leo42 is unable to grow anaerobically in the medium used to assay AdhE.