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Alleviating ability of hirudin on the renal fibrosis of rat. (a) HE staining and Masson staining. Scale bar: 50 μm. (b) Scoring on renal tubule injury condition. (c) Fibrotic area on fibrosis condition. (d) Evaluation on Scr content. (e) Western blot detection to reveal the expressions of fibronectin, collagen III, and α-SMA. (f) Semiquantitative analysis was performed based on the gray value. GAPDH was used for the endogenous protein. ##compared with UUO, P < 0.01.
