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Construction and isolation of S. mutans G9IS1A and IS1A using pGh9:ISS1 and isolation of plasmids carrying DNA flanking the ISS1 element in IS1A. Thick lines and arrowheads represent genomic DNA, thin lines represent plasmid DNA, and small arrowheads represent ISS1 DNA. Abbreviations for restriction enzymes: E, EcoRI; H, HindIII.
