FIG. 3.

Physical map and ORF characterization of 11,202 bp of the S. mutans LT11 genome based on DNA sequence analysis of pIS1A/E:r, pIS1A/H:r (plasmids rescued from strain IS1A carrying the flanking ends of the transposon and their junction sites), and the PCR product (hatched bar) produced using primers of the adjacent S. mutans DNA. The MunI/NspV fragment that was disrupted by insertion of an Em^r gene and used to construct pDLTC-Em is also shown. Hairpin structures represent putative transcriptional terminators. Genetic designations: ytqB, unknown; abcX, ABC transport protein; perM, permease; hlyX, hemolysin; pflC, pyruvate-formate lyase activase; dltABCD, genes of the dlt operon; ppx1, exopolyphosphatase. Abbreviations for restriction sites: B, BglII; E, EcoRI; H, HindIII; M, MunI; N, NspV; P, PstI, R, RcaI; S, SphI; X, XbaI.