FIG. 5.
Lysis phenotype of SA52G, S107A52G, and S107C51S. In all panels, lysogens carrying the specified plasmids were induced and monitored for turbidity. (A) Lysogen, MC4100(λCmΔSR); plasmids, pKB110 (S+) (○), pS105 (□), pSA52G (●), or pS105A52G (■). (B) Lysogen, MC4100(λCmΔSR); plasmids, pKB1 (Sam7) (○), pS107 (□), pS107C51S (●), or pS107A52G (■). (C) Lysogen, MC4100(λKnΔSR); plasmids, pKB1 (Sam7) (○), pS107 (□), or pS107C51S (●). In this experiment the cultures were divided into two flasks 90 min after induction and 10 mM KCN was added to one flask (dashed lines). The turbidity of the culture was monitored until lysis was completed or 130 min after induction.