Fig. 2.
Characteristics of dendritic cell-mimicking scaffolds. (a) (Left) CLSM images (scale bar, 50 μm) and (right) TEM images (scale bar, 500 nm) of bare MF and DC membrane-coated MF (DC@MF). For CLSM, DC membrane was stained with Dil dye (Dil-CM, red). (b) Zeta-potential of bare MF, DC membrane, and DC@MF (***p < 0.001; one-way ANOVA followed by Tukey's post hoc tests). (c) UV–Vis spectra of antibodies functionalized with DBCO groups (λDBCO = 317 nm, arrow). (d) Fluorescence microscopy images of DC@MF decorated with αCD3 or αCD28 (scale bar, 100 μm). The αCD3 or αCD28 mouse antibodies decorated on DC@MF were detected and visualized by an anti-mouse secondary antibody labeled with Alexa Flour® 488 (green). (e) Decoration of various inputs of DBCO-functionalized antibodies onto 100 μg DC@MF. (f) Formulations of IL-2/Ab/DC@MF for culture of 106 cells. (g) IL-2 release profile of IL-2/Ab/DC@MF.