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. 2022 Sep 11;21:241–252. doi: 10.1016/j.bioactmat.2022.08.015

Table 1.

Formulations of Dynabead and IL-2/Ab/DC@MF for polyclonal T cell expansion.

For 106 cell culture Cell-to-bead ratio Additional amount Antibody presented
IL-2 loaded (nM) IL-2 supplemented in media (nM)
Total amount (μg) Surface density (pmol/cm2) Conc. (nM)
Dynabeada 1:0.05 5000 ea 0.4 868 6.9 1.3
1:1 106 ea 82.8 1393.9
IL-2/Abb/DC@MF 100 μg 0.4 2.1 6.4 58
a

Dynabeads functionalized with αCD3 and αCD28.

b

1:2 M ratio of αCD3:αCD28 for decoration.