Table 1.
For 106 cell culture | Cell-to-bead ratio | Additional amount | Antibody presented |
IL-2 loaded (nM) | IL-2 supplemented in media (nM) | ||
---|---|---|---|---|---|---|---|
Total amount (μg) | Surface density (pmol/cm2) | Conc. (nM) | |||||
Dynabeada | 1:0.05 | 5000 ea | 0.4 | 868 | 6.9 | – | 1.3 |
1:1 | 106 ea | 82.8 | 1393.9 | – | |||
IL-2/Abb/DC@MF | – | 100 μg | 0.4 | 2.1 | 6.4 | 58 |
Dynabeads functionalized with αCD3 and αCD28.
1:2 M ratio of αCD3:αCD28 for decoration.