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Ni2+-citrate uptake and citM promoter activity. Uptake of [1,5-14C]citrate in whole cells of B. subtilis 168 grown in CSE medium without further additions (□, CSE), with 10 mM citrate (○, CSEC), and with 10 mM citrate plus 10 mM glucose (▵, CSECG). (Inset) β-Galactosidase activity (in Miller units [MU]) of B. subtilis CM002 carrying the lacZ gene under the control of the citM promoter grown in the same media.
