FIG. 3.

Effect of different growth substrates on transport and promoter activities. B. subtilis strains 168 and CM002 were grown in C minimal medium with 10 mM citrate in the presence of no further additions (none), glucose (Gluc), glycerol (Gly), inositol (I), inositol and glutamate (IE), and succinate and glutamate (SE). Open bars, initial rates of uptake of [1,5-¹⁴C]citrate in the presence of 1 mM NiCl₂ by whole cells of B. subtilis 168; solid bars, β-galactosidase activity of B. subtilis CM002 grown in the different media in Miller units (MU).