TABLE 1.
B. subtilis strains used in this study
| Strain | Genotypea | Source (reference[s]) |
|---|---|---|
| 168 | trpC2 | Microbiology, University of Groningen |
| SA003 | trpC2 sacB′-′lacZ ptsH1 | J. Deutscher (7) |
| QB7097 | trpC2 crh::spc | I. Martin-Verstraete |
| QB7102 | trpC2 ptsH1 crh::aphA3 | I. Martin-Verstraete (14, 34) |
| QB5407 | trpC2 ccpA::Tn917 spc | I. Martin-Verstraete (10) |
| CM002 | trpC2 amyE::(PcitM-lacZ cat) | This study |
| CM004 | trpC2 sacB′-′lacZ ptsH1 amyE::(PcitM- lacZ cat) | This study |
| CM006 | trpC2 crh::spc amyE::(PcitM-lacZ cat) | This study |
| CM008 | trpC2 ptsH1 crh::aphA3 amyE::(PcitM- lacZ cat) | This study |
| CM010 | trpC2 ccpA::Tn917 spc amyE::(PcitM- lacZ cat) | This study |
a
Tn917 spc, Tn917 derivative conferring resistance to spectinomycin; aphA3, Enterococcus faecalis kanamycin resistance gene; cat pC194 chloramphenicol acetyltransferase gene.