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. 2022 Sep 14;13:5383. doi: 10.1038/s41467-022-32841-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Photo-triggered assembly of a transient MHC I–tapasin–ERp57 complex (black) analyzed by size exclusion chromatography (SEC). SEC elution profiles of tapasin–ERp57 and MHC I hc–β₂m are colored in orange and teal, respectively. β₂m β₂-microglobulin; hc heavy chain; A₂₈₀ absorption at 280 nm; V₀ void volume; V_t total volume. b MHC I–tapasin–ERp57 complex formation monitored by SEC and SDS-PAGE. kDa kilodalton; n = 1. Same color code as illustrated in a is used. c Cartoon representation of peptide-receptive MHC I (MHC I hc, teal; β₂m, green) in complex with the ER chaperone tapasin–ERp57 (tapasin, orange; ERp57, red) in different orientations. a, b, b′, and a′, thioredoxin-like domains of ERp57. Source data for a and (b) are provided as a Source Data file.