KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-biotin PE clone 1D4-C5	Biolegend	Cat# 409004; RRID: AB_10641847
Anti-biotin	Abcam	Cat# ab53494; RRID: AB_867860
Sepharose anti-GFP antibody	Abcam	Cat# ab69314; RRID: AB_1640178
Anti-GFP	Thermo Fisher	Cat# A-11122; RRID: AB_221569
Anti-rabbit IgG HRP-conjugated	R&D systems	Cat# HAF008; RRID: AB_357235
Rat monoclonal anti-mouse Ly6G clone 1A8	BioXCell	Cat# BE0075-1; RRID: AB_1107721
Rat monoclonal IgG2a isotype control clone 2A3	BioXCell	Cat# BE0089; RRID: AB_1107769
Anti-mouse CD45 APC-Cy7 clone 30-F11	Biolegend	Cat# 103116; RRID: AB_312981
Anti-mouse CD11b PE/Dazzle594 Clone M1/70	Biolegend	Cat# 101256; RRID: AB_2563648
Anti-mouse CD11c PE/Cy7 Clone N418	Biolegend	Cat# 117318; RRID: AB_493568
Anti-mouse Ly6C APC Clone HK1.4	Biolegend	Cat# 128016; RRID: AB_1732076
Anti-mouse Ly6G BV421 Clone 1A8	Biolegend	Cat# 127628; RRID: AB_2562567
Anti-mouse F4/80 BV605 Clone BM8	Biolegend	Cat# 123133; RRID: AB_2562305
Anti-mouse Siglec F BB515 Clone E50-2440	BD Bioscience	Cat# 564514; RRID: AB_2738833
Anti-mouse c-Kit BV421 Clone ACK2	Biolegend	Cat# 135124; RRID: AB_2562237
Anti-mouse ICAM FITC Clone YN/1.7.4	Biolegend	Cat# 116105; RRID: AB_313696
Anti-mouse F4/80 PE Clone BM8	Biolegend	Cat# 123110; RRID: AB_893486
Anti-mouse FcεRI PE/Cy7 Clone MAR-1	Biolegend	Cat# 134318; RRID: AB_10640122
Anti-mouse CD11b BV785 Clone M1/70	Biolegend	Cat# 101243; RRID: AB_2561373
Anti-mouse CD11c BV605 Clone N418	Biolegend	Cat# 117334; RRID: AB_2562415
Anti-mouse I-A/I-E BV711 Clone M5/114.15.2	Biolegend	Cat# 107643; RRID: AB_2565976
Anti-mouse Ly6G PE/Cy7 Clone 1A8	Biolegend	Cat# 127617; RRID: AB_1877262
Anti-mouse CD11b PE Clone M1/70	Biolegend	Cat# 101207; RRID: AB_312790
Anti-mouse B220 APC Clone RA3-6B2	eBioscience	Cat# 17-0452-82; RRID: AB_469395
Anti-mouse Ly6G AF700 Clone 1A8	Biolegend	Cat# 127622; RRID: AB_10643269
Anti-mouse CD49b PE Clone DX5	Biolegend	Cat# 108908; RRID: AB_313415
Rabbit anti-mouse BD14	MyBioSource	Cat# MBS1490249; RRID: AB_2732866
Rat anti-mouse Ly6G	Biolegend	Cat# 127602; RRID: AB_1089180
Rabbit anti-S. aureus	Abcam	Cat# 20920;RRID: AB_445913
Alexa Fluor ® 488 goat anti-rabbit IgG	Thermo Fisher	Cat# A-11008; RRID: AB_143165
Alexa Fluor ® 488 goat anti-rat IgG	Thermo Fisher	Cat# A-11006; RRID: AB_2534074
Alexa Fluor ® 594 goat anti-rabbit IgG	Thermo Fisher	Cat# A-11012; RRID: AB_2534079
Goat anti-mouse IL-1β	R&D systems	Cat# AF-401-NA; RRID: AB_416684
Rabbit anti-cleaved-IL-1β	Cell Signaling Technology	Cat# 63124s
Rabbit anti-goat IgG HRP-conjugated	R&D systems	Cat# HAF017; RRID: AB_562588
HRP anti-beta Actin	Abcam	Cat# Ab49900; RRID: AB_867494
Bacterial and virus strains
Staphylococcus aureus SF8300	Binh Diep (UCSF)	N/A
USA300	Tammy Kielian (University of Nebraska)	N/A
Chemicals, peptides, and recombinant proteins
hBD3	Anaspec	Cat# AS-60741
Biotin-hBD3	Anaspec	N/A
mBD14	Wuyuan Lu (University of Maryland Baltimore)	N/A
mBD3	Wuyuan Lu (University of Maryland Baltimore)	N/A
S. aureus derived LTA	Invivogen	Cat# Tlrl-pslta
Tryptic soy broth	Millipore	Cat# 22092
TRIzol reagent	Thermo Fisher	Cat# 15596018
Liberase TL	Roche	Cat# 05401054001
DNase I	Sigma-Aldrich	Cat# 4536282001
Recombinant IL-1β	R&D systems	Cat# 401-ML
NuPAGE™ LDS Sample Buffer	Thermo Fisher	Cat# NP0007
Q-VD-Oph	R&D systems	Cat# OPH001-01M
YM254890	Tocris	Cat# 7352
SYTOX blue	Thermo Fisher	Cat# S34857
SYTOX red	Thermo Fisher	Cat# S34859
SYTOX orange	Thermo Fisher	Cat# S11368
Critical commercial assays
Mouse Elastase DuoSet ELISA Kit	R&D systems	Cat# DY4517-05
Mouse MPO DuoSet ELISA Kit	R&D systems	Cat# DY3667
Mouse S100A8/A9 DuoSet ELISA Kit	R&D systems	Cat# DY8596-05
Mouse IL-1β DuoSet ELISA Kit	R&D systems	Cat# DY401-05
Mouse Cxcl2 DuoSet ELISA Kit	R&D systems	Cat# DY452-05
Mouse BD14 ELISA Kit	MyBioSource	Cat# MBS2515829
MACS neutrophil isolation kit	Miltenyi Biotec	Cat# 130-097-658
RNAscope® Multiplex Fluorescent Detection Kit v2	ACDBio	Cat# 323110
RNAscope® Ly6g probe	ACDBio	Cat# 455701
RNAscope® Mrgpra2 probe	ACDBio	Custom-made
RNeasy Plus Mini kit	Qiagen	Cat# 74034
Chemotaxis assay kit	Abcam	Cat# Ab235692
Cellular reactive oxygen species detection assay kit	Abcam	Cat# Ab113851
Deposited data
RNA-seq data	NCBI GEO	GSE178507
Experimental models: Cell lines
Gα15 HEK 293 cells	This study	N/A
Mrgpra2b-GFP; Gα15 HEK 293 cells	This study	N/A
MRGPRX3-FLAG; Gα15 HEK 293 cells	This study	N/A
MRGPRX4-GFP; Gα15 HEK 293 cells	This study	N/A
Experimental models: Mice
C57BL/6J	Jackson laboratory	Cat# 000664
Mrgpra2 dKO	This study	N/A
K14-cre; Defflox/flox	This study	N/A
K14-cre	Jackson laboratory	Cat# 018964
Oligonucleotides
gRNAs targeting Mrgpra2a/b CAAGTTAGTTTTGGTCAATC, GATCTGATACCCTCTTCTGG, TAAACTGGCTATCTACTTGA, TTATCCAATGCTGGGATAAT	This study	N/A
Genotyping primers for Mrgpra2 dKO GTCAGCATGATGGTCAC (WT-F), CATAGGGAAGGCCTCATGTCTC (WT-R), CTATGGAGGGATGCTCATTACTG (Mrgpra2 dKO-F), CAAGATCTGACTCCCTCTTCTGG (Mrgpra2 dKO-R)	This study	N/A
gRNA targeting Defb13 CCAAGCCAATTTCCTGTGCTGCA	This study	N/A
gRNA targeting Defb40 CCATGGGCATTTCTGAATGTGAC	This study	N/A
Primers for genotyping Def cluster deletion GATGCAGGTGCCCTGAAATAT (F) CACTGAATAGACCTGACTTCC (R)	This study	N/A
RT-PCR primer for Mrgpra2 CCTCCTACACAAGCCAGCAA (F) AAGCACAAGTGAAAGATGATGCT	This study	N/A
RT-PCR primer for Gapdh TGTTCCTACCCCCAATGTGT (F) TGTGAGGGAGATGCTCAGTG (R)	Zambrano et al., 2019	N/A
Software and algorithms
FlowJo	BD	https://www.flowjo.com/
GraphPad Prism 9	GraphPad	https://www.graphpad.com/scientific-software/prism/
ImageJ	NIH	https://fiji.sc/
R	R	https://www.r-project.org/
QIIME2	QIIME2 development team	https://qiime2.org/
STAR - 2.7.3a	Alexander Dobin	https://github.com/alexdobin/STAR/releases/tag/2.7.9a
Partek Flow	Partek	https://www.partek.com/
Partek GS v7.0	Partek	https://www.partek.com/
Spotfire	TIBCO	https://www.tibco.com/products/tibco-spotfire